The gelling properties of Dillenia indica mucilage in benzyl benzoate emulgel formulations

Abstract The objective of the study was to evaluate the gelling properties of Dillenia indica mucilage in benzyl benzoate emulgel formulation. Mucilage was extracted from the fruits of Dillenia indica using established methods and characterized by rheology and swelling. Emulsion (F1) was prepared using the continental emulsification method. Gelling agents (2 %w /v) were prepared by dispersing in distilled water with constant stirring at a moderate speed using a magnetic stirrer. F1 was added to the gel (0-75 %w /w) to obtain emulgel formulations and evaluated using viscosity, globule size, pH, release profiles and kinetic modeling. Data were expressed as mean ± SD, and similarity factor (f2) was used to compare all formulations. Formulation viscosity was significantly higher with carbopol than with Dillenia; globule sizes increased with concentration of gelling agents, and pH reduced as the concentration of Dillenia increased. All formulations showed controlled release properties with t80 ranging between 114 and 660 min. The release was governed by Korsmeyer-Peppas model. Formulation F5 prepared with 50 % Dillenia showed highest similarity to F4 prepared with 75 %w /w carbopol. Dillenia indica demonstrated acceptable gelling properties comparable with that of carbopol and could be improved for use in emulgel formulations.

Development and in vitro/in vivo evaluation of thermo-sensitive in situ gelling systems for ocular allergy

Ocular allergy is one of the most common disorders of the eye surface. Following diagnosis this condition is typically treated with preparations containing antihistamines. However, anatomy of the eye and its natural protective mechanisms create challenges for ocular drug delivery. Rapid elimination of antihistamine substances due to short residency times following application can lead to insufficient treatment of ocular allergies. With this in mind, the aim of this study was to prepare a controlled ocular delivery system to extend the retention time of olopatadine hydrochloride (OLO) and in doing so to reduce the need for frequent application. We developed extended-release ocular in situ gelling systems for which in vivo retention times were determined in sheep following in vitro characterization and cytotoxicity studies. In vivo results were then compared to commercially available Patanol eye drops. the transparent gels formulated using appropriate amounts of polymers and having longer ocular retention times appear to be a viable alternative to commercially available eye drops.

Caracterización de una jalea de Calendula officinalis L. al 1% para uso estomatológico / Characterization of a jelly of Calendula officinalis L. at 1% for stomatological use

Introducción: La Calendula officinalis L. es una de las especies vegetales más utilizadas en el tratamiento de enfermedades periodontales. Objetivo: Determinar preliminarmente los parámetros de calidad de la jalea de Calendula officinalis L. al 1% para uso estomatológico. Métodos: Se elaboraron 18 formulaciones utilizando como agente gelificante carboximetilcelulosa (CMC) e hidroxipropilmetilcelulosa (HPMC), empleando el método de incorporación. A las mismas se le determinaron los parámetros de calidad y se seleccionaron las cuatro con mejores parámetros tecnológicos para evaluar el grado de aceptación sensorial por parte de un comité de expertos. Resultados: Las formulaciones con HPMC fueron las que presentaron mejores características organolépticas y pH aceptable para su aplicación sobre la mucosa bucal; así como un área de extensibilidad óptimo, lo que permitió que fueran seleccionadas para realizar el análisis sensorial. Conclusión: Se establecieron preliminarmente los parámetros de calidad de la jalea Calendula officinalis L. al 1% siendo seleccionada la formulación codificada como HPMC 6 por presentar olor característico al extracto blando, color ámbar tenue, homogéneo y con brillo, sabor agradable, no presentar grumos ni arenosidad, área de extensibilidad de 66.96±0.91cm2, pH de 4.9 y mayor aceptabilidad sensorial

Introduction: Calendula officinalis L. is one of the plant species most used in the treatment of periodontal diseases. Objective: To determine preliminarily the quality parameters of Calendula officinalis L. jelly at 1% for stomatological use. Methods: 18 formulations were prepared using carboxymethylcellulose (CMC) and hydroxypropylmethylcellulose (HPMC) as the gelling agent, using the incorporation method. The quality parameters were determined selecting the four formulations with better technological parameters to evaluate sensory acceptance by a committee of experts. Results: The HPMC formulations presented better organoleptic characteristics and acceptable pH for its application on the buccal mucosa; as well as an area of optimal extensibility, which allowed them to be selected to carry out the sensory analysis. Conclusion: The quality parameters of the Calendula officinalis L. 1% jelly were preliminarily established the formulation encoded as HPMC 6 is selected for presenting characteristic odor to the soft extract, amber color subdued, homogeneous and glossy, pleasant taste, no lumps or grit, area of extensibility of 66.96 ± 0.91cm2, pH of 4.9 and greater sensory acceptability

Uso de apósitos con tecnología alveolar gelificante para cura de úlceras por presión / Usage of dressings with gelifying alveolar technology for pressure ulcer healing

Objetivo: Describir el proceso de cicatrización de dos úlceras por presión con el uso de apósitos con tecnología alveolar gelificante. Metodología: aplicación de las guías vigentes de tratamiento de las úlceras por presión. Resultados: Ambas úlceras evolucionaron progresivamente de manera favorable hasta conseguir su cicatrización a los 3 meses del inicio del tratamiento. Conclusiones: El uso de apósitos con tecnología alveolar gelificante, unido a la aplicación de las medidas preventivas, posturales e higiénico-dietéticas recomendadas en las guías de tratamiento de las úlceras por presión, fueron determinantes para el buen desarrollo del proceso de cicatrización de las lesiones

Aim: The aim was to describe the healing process of two pressure ulcers with the usage of dressings with gelifying alveolar technology. Methodology: Application of the current guidance about pressure ulcers treatment. Results: Both ulcers evolved progressively and favorably until reach their healing in three months from the beginning of the treatment. Conclusions: The usage of dressing with gelifying alveolar technology along with the application of preventive, postural and hygienicdietetic measures recommended by the guidance of pressure ulcers treatment, were essential for the good development of the healing process

Tratamiento de una úlcera venosa con terapia compresiva multicapa y tecnología alveolar gelificante / Treatment of a venous ulcer with multilayer compression therapy and alveolar gelling technology

Varón de 49 años, que presentó una úlcera venosa en maléolo interno de la extremidad inferior derecha de más de 2 años de evolución. En el caso clínico se exponen las pautas de tratamiento utilizadas, la evolución de dicha lesión y las medidas de prevención recomendadas una vez cicatrizada. Debido a que las úlceras venosas tienen una elevada prevalencia, que se sitúa entre el 70% y el 80%1 del total de las heridas en extremidades inferiores, lo que conlleva un elevado gasto sanitario, creemos importante la realización del índice tobillo-brazo, así como la correcta utilización de los tratamientos tópicos

A 49 years old male presented a venous ulcer on the internal malleolus of the right lower extremity of more than 2 years of evolution. In the clinical case, the treatment guidelines used, the evolution of the injury and the recommended prevention measures once healed are exposed. Because venous ulcers have a high prevalence between 70-80% of the total wounds in the lower extremities, which entails a high health expenditure, this is because we believe that the anklebrachial index is important, as well as the correct use of topical treatments

Production and characterization of alginate-starch-chitosan microparticles containing stigmasterol through the external ionic gelation technique

Stigmasterol - a plant sterol with several pharmacological activities - is susceptible to oxidation when exposed to air, a process enhanced by heat and humidity. In this context, microencapsulation is a way of preventing oxidation, allowing stigmasterol to be incorporated into various pharmaceutical forms while increasing its absorption. Microparticles were obtained using a blend of polymers of sodium alginate, starch and chitosan as the coating material through a one-stage process using the external gelation technique. Resultant microparticles were spherical, averaging 1.4 mm in size. Encapsulation efficiency was 90.42% and method yield 94.87%. The amount of stigmasterol in the oil recovered from microparticles was 9.97 mg/g. This technique proved feasible for the microencapsulation of stigmasterol.

O estigmasterol, um fitoesterol com diversas atividades farmacológicas, é suscetível à oxidação quando exposto ao ar, calor e umidade. Neste contexto, a microencapsulação é uma forma de proteção contra oxidação, permitindo a incorporação do estigmasterol em diversas formas farmacêuticas e aumentar sua absorção. As micropartículas foram obtidas por gelificação iônica externa, em uma etapa, utilizando como revestimento polímeros naturais de alginato de sódio, amido de milho e quitosana. As micropartículas apresentaram formato esférico com tamanho aproximado de 1,4 mm. O rendimento foi de 94,87% e a eficiência média de encapsulação de 90,42%. A quantidade de estigmasterol no óleo recuperado das micropartículas foi de 9,97 mg/g. O método mostrou-se viável para a microencapsulação do estigmasterol.

Effect of cross-linked biodegradable polymers on sustained release of sodium diclofenac-loaded microspheres

The objective of this study was to formulate an oral sustained release delivery system of sodium diclofenac(DS) based on sodium alginate (SA) as a hydrophilic carrier in combination with chitosan (CH) and sodium carboxymethyl cellulose (SCMC) as drug release modifiers to overcome the drug-related adverse effects and to improve bioavailability. Microspheres of DS were prepared using an easy method of ionotropic gelation. The prepared beads were evaluated for mean particle size, entrapment efficiency, swelling capacity, erosion and in-vitro drug release. They were also subjected to various studies such as Fourier Transform Infra-Red Spectroscopy (FTIR) for drug polymer compatibility, Scanning Electron Microscopy for surface morphology, X-ray Powder Diffraction Analysis (XRD) and Differential Scanning Calorimetric Analysis (DSC) to determine the physical state of the drug in the beads. The addition of SCMC during the preparation of polymeric beads resulted in lower drug loading and prolonged release of the DS. The release profile of batches F5 and F6 showed a maximum drug release of 96.97 ± 0.356% after 8 h, in which drug polymer ratio was decreased. The microspheres of sodium diclofenac with the polymers were formulated successfully. Analysis of the release profiles showed that the data corresponds to the diffusion-controlled mechanism as suggested by Higuchi.

O objetivo deste estudo foi elaborar um sistema de entrega de oral de liberação sustentada de diclofenaco sódico (DS) com base em alginato de sódio (SA), como um transportador hidrofílico em combinação com quitosana (CH) e carboximetilcelulose de sódio (SCMC) como modificadores de liberação de fármaco para diminuir os efeitos adversos e melhorar a biodisponibilidade. Prepararam-se microesferas de DS usando um método fácil de geleificação ionotrópica. Avaliaram-se os grânulos preparados quanto ao tamanho médio de partícula, eficiência de compressão, inchaço in vitro, erosão e capacidade de liberação de fármacos. Estes tammbém foram submetidos a vários estudos, como espectrometria no infravermelho com transformada de Fourier (FTIR) para compatibilidade de fármaco e polímero, microscopia eletrônica de varredura para morfologia de superfície, análise de difração de raios-X (XRD) do pós e análise calorimétrica diferencial de varredura (DSC) para determinar o estado físico do fármaco nos grânulos. A adição de SCMC durante a preparação de grânulos do polímero resultou em fármacos com menor carga de fármaco e liberação prolongada do DS. O perfil de liberação dos lotes F5 e F6 apresentou máximo de fármaco liberado de 96,97±0,356% após 8 h após o que a proporção do fármaco no polímero foi diminuída. As microesferas de diclofenaco de sódio com os polímeros foram formuladas com sucesso. A análise dos perfis de liberação mostrou que os dados correspondem ao mecanismo de difusão controlada, como sugerido por Higuchi.

Development and evaluation of calcium alginate beads prepared by sequential and simultaneous methods

The objective of this study was to develop a sustained release dosage form of Trimetazidine dihydrochloride (TMZ) using a natural polymeric carrier prepared in a completely aqueous environment. TMZ was entrapped in calcium alginate beads prepared with sodium alginate by the ionotropic gelation method using calcium chloride as a crosslinking agent. The drug was incorporated either into preformed calcium alginate gel beads (sequential method) or incorporated simultaneously during the gelation stage (simultaneous method). The beads were evaluated for particle size and surface morphology using optical microscopy and SEM, respectively. Beads produced by the sequential method had higher drug entrapment. Drug entrapment in the sequential method was higher with increased CaCl2 and polymer concentration but lower with increased drug concentration. In the simultaneous method, drug entrapment was higher when polymer and drug concentration were increased and also rose to a certain extent with increase in CaCl2 concentration, where further increase resulted in lower drug loading. FTIR studies revealed that there is no interaction between drug and CaCl2. XRD studies showed that the crystalline drug changed to an amorphous state after formulation. Release characteristics of the TMZ loaded calcium alginate beads were studied in enzyme-free simulated gastric and intestinal fluid.

O objetivo deste estudo foi desenvolver forma de liberação controlada de dicloridrato de trimetazidina (TMZ) utilizando transportador plomérico natural em ambiente completamente aquoso. A TMZ foi presa em pérolas de alginato de cálcio preparadas com alginato de sódio pelo método de gelatinização ionotrópica, usando cloreto de cálcio como agente de formação de ligações cruzadas. O fármaco foi incorporado nas pérolas de gel de alginato de cálcio (método sequencial) ou incorporado, simultaneamente, durante o estágio de gelificação (método simultâneo). As pérolas foram avaliadas quanto ao tamanho das partículas e morfologia da superfície utilizando microscopia óptica de SEM, respectivamente. As pérolas produzidas pelo método sequencial apresentaram maior capacidade de inclusão. No método sequencial, a inclusão de fármaco foi maior com o aumento de CaCl2 e da concentração do plímero, mas menor com o aumento da concentração de fármaco. No método simultâneo, a inclusão de fármaco foi mais alta quando as concentrações de fármaco e plímero foram aumentadas e, também, atingiram certa extensão com aumento na concentração de CaCl2, cujo aumento posterior resultou em carga menor de fármaco. Estudos de FTIR revelaram que não há interação entre fármaco e CaCl2. Estudos de XRD mostraram que o fármaco mudou do estado cristalino para o amorfo após a formulação. As características de liberação de TMZ das pérolas carregadas com alginato de cálcio foram estudadas em fluidos simulados, gástrico e intestinal, livres de enzima.

Development, stability and in vitro permeation studies of gels containing mometasone furoate for the treatment of dermatitis of the scalp / Estabilidade, desenvolvimento e in vitro estudos de permeação de gel contendo furoato de mometasona para o tratamento da dermatite do couro cabeludo

Dermatological inflammatory diseases such as atopic dermatitis, psoriasis and seborrhoeic dermatitis often affect the scalp and the eyebrows. Although there are many dosage forms available, these are particularly critical anatomic regions for application of topical formulations because of the presence of hair. Lotions are therefore the recommended type of drug delivery system for these areas. The presence of hair may limit the application and thus the acceptability of the formulation and its compliance. Because of its low apparent viscosity, lotion application is unpleasant. Gels, given their consistency and adhesiveness, are a suitable alternative to lotions in this situation. The aim of this study was to formulate a stable gel containing mometasone furoate, which is an anti-inflammatory and anti-pruritic corticosteroid, in order to improve topical treatment of scalp dermatitis. In this study, pharmaceutical development, physical-chemical characterization, stability and in vitro permeation studies were performed. In terms of the pH, viscosity, assay and macroscopic and microbiological properties, the gel was stable over the period of study. The in vitro permeation studies allowed the characterization of the mometasone furoate permeation profile for the gel through different membranes. Mometasone furoate presented a slow permeation through the skin. This gel appears safe for topical application.

Afecções dermatológicas do tipo inflamatório como a dermatite atópica, psoríase e dermatite seborreica, afetam freqüentemente o couro cabeludo e sobrancelhas. Apesar de existirem várias formas farmacêuticas para o seu tratamento, apenas as loções são indicadas para estas zonas, mas devido à baixa viscosidade, a aplicação de loções torna-se desagradável. Os geles, pela maior consistência e capacidade de adesão, apresentam-se como alternativa nesta situação. Neste trabalho procedeu-se ao desenvolvimento galênico de um gel com furoato de mometasona, que é um potente corticóide de última geração, com um rácio melhorado de risco/benefício. Foram avaliadas as características físico-químicas, a estabilidade e foram realizados ensaios de permeação in vitro. O gel obtido apresenta características organolépticas e reológicas adequadas ao fim a que se destina tendo-se apresentado estável química, física e microbiologicamente durante o tempo do ensaio. Os estudos de permeação in vitro permitiram caracterizar a formulação através de diferentes membranas. A membrana biológica (pele) não permite uma grande permeação do fármaco o que poderá sugerir que esta formulação é segura para aplicação tópica.

Técnicas de microencapsulación: una propuesta para microencapsular probióticos / No disponible

A la hora de utilizar probióticos, el principal problema que se presenta, es la escasa resistencia de estos a diferentes condiciones ambientales y tecnológicas. Las técnicas de microencapsulación son un buen método para proteger a estos microorganismos, sin embargo no todas las técnicas son apropiadas para los probióticos. En este artículo proponemos la técnica de gelificación interna, que por sus características permite la obtención de un tamaño de partícula adecuado y la supervivencia de los microorganismos(AU)

The main problem when probiotics are used is the low resistance of these to different environmental and technological conditions. The microencapsulation techniques are a good method in order to protect the probiotics, Nevertheless not all techniques of microencapsulation are suitable for probiotics. In this paper, we propose the internal gelification which allows us to obtain a suitable particle size and the survival of the microorganisms(AU)